{"id":18742,"date":"2025-07-31T19:43:00","date_gmt":"2025-07-31T12:43:00","guid":{"rendered":"https:\/\/fpik.unpad.ac.id\/?p=18742"},"modified":"2025-09-18T19:45:26","modified_gmt":"2025-09-18T12:45:26","slug":"exploring-plastic-degrading-enzyme-producers-from-ascidian-associated-bacteria-in-karimunjawa-islands-indonesia","status":"publish","type":"post","link":"https:\/\/fpik.unpad.ac.id\/en\/exploring-plastic-degrading-enzyme-producers-from-ascidian-associated-bacteria-in-karimunjawa-islands-indonesia\/","title":{"rendered":"Exploring plastic-degrading enzyme producers from Ascidian-associated bacteria in Karimunjawa Islands, Indonesia"},"content":{"rendered":"<p class=\"wp-block-paragraph\">Plastic pollution has become one of the world\u2019s most urgent environmental problems, with marine ecosystems facing severe impacts from waste accumulation. This study focused on exploring bacteria that live in association with <strong>ascidians<\/strong>\u2014marine invertebrates commonly found on coral reefs and marine debris\u2014in the <strong>Karimunjawa Islands, Indonesia<\/strong>. The research aimed to identify bacteria capable of producing enzymes, such as <strong>PETase<\/strong>, that can degrade plastics.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Samples from nine ascidian species, including <em>Eusynstyela sp.<\/em>, <em>Didemnum sp.<\/em>, and <em>Phallusia sp.<\/em>, were collected from depths of 5\u201320 meters. Using a combination of microbiological and molecular techniques, researchers successfully isolated <strong>45 bacterial strains<\/strong>. These strains were tested for their ability to break down plastic, specifically low-density polyethylene (LDPE).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">The findings revealed that about <strong>33% of the isolates showed enzymatic activity<\/strong>, and two isolates demonstrated significant plastic-degrading potential. Molecular analysis through <strong>16S rRNA gene sequencing<\/strong> identified these as <em>Staphylococcus condimenti<\/em> (a Gram-positive bacterium often found in fermentation processes) and <em>Alcanivorax dieselolei<\/em> (a Gram-negative bacterium known for breaking down hydrocarbons and commonly associated with oil spill bioremediation).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Scanning Electron Microscope (SEM) analysis confirmed that these bacteria were capable of colonizing plastic surfaces, forming <strong>biofilms<\/strong>, and causing structural degradation of the plastic material. Although the degradation rates were modest compared to some insect-associated microorganisms, the study emphasized that optimizing environmental conditions (temperature, pH, nutrient composition) could enhance bacterial performance.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">This research is groundbreaking because it demonstrates that ascidian-associated bacteria in tropical marine environments may serve as <strong>natural resources for biotechnological solutions<\/strong> to plastic pollution. Unlike genetic engineering approaches that are costly and often laboratory-bound, these marine bacteria could provide eco-friendly alternatives to reducing persistent plastic waste in the ocean.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Credits<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Meet the Researchers: This collaborative study was led by <strong>Diah Ayuningrum<\/strong> (Universitas Diponegoro), with contributions from <strong>Moh Yunus Shukor<\/strong> (Universiti Putra Malaysia), <strong>Mufti Petala Patria<\/strong> (Universitas Indonesia), <strong>Buntora Pasaribu<\/strong> (Universitas Padjadjaran), and <strong>Sulistiowati<\/strong> (Universitas Gadjah Mada). The project was supported by <strong>Riset Kolaborasi Indonesia 2024<\/strong><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Hashtags<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">#MarineBiotech #PlasticPollution #SDG14 #BluePlanetSolutions #OceanScience #Biodegradation<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>Publication Link<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/smujo.id\/biodiv\/article\/view\/20435\">https:\/\/smujo.id\/biodiv\/article\/view\/20435<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>","protected":false},"excerpt":{"rendered":"<p>Plastic pollution has become one of the world\u2019s most urgent environmental problems, with marine ecosystems facing severe impacts from waste accumulation. This study focused on exploring bacteria that live in association with ascidians\u2014marine invertebrates commonly found on coral reefs and marine debris\u2014in the Karimunjawa Islands, Indonesia. The research aimed to identify bacteria capable of producing [&hellip;]<\/p>\n","protected":false},"author":1001026,"featured_media":18745,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[68],"tags":[],"class_list":["post-18742","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sdgs-papers"],"_links":{"self":[{"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/posts\/18742","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/users\/1001026"}],"replies":[{"embeddable":true,"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/comments?post=18742"}],"version-history":[{"count":1,"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/posts\/18742\/revisions"}],"predecessor-version":[{"id":18746,"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/posts\/18742\/revisions\/18746"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/media\/18745"}],"wp:attachment":[{"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/media?parent=18742"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/categories?post=18742"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fpik.unpad.ac.id\/en\/wp-json\/wp\/v2\/tags?post=18742"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}